For functional unit determination, the Shimadzu Fourier transform Infrared Spectrophotometer- FTIR 8400 S was used. Samples were weigh in at 0.01 g and homogenized with 0.01 g KBr anhydrous by mortar agate. The mixtures were pressed by vacuum hydraulic (Graseby Specac) at 1.2 psi to obtain transparency pellet. Scanned sample passed through infra red, where its continuing wave by detector that connected to computer and given described of tested sample spectrum. Samples were usually scanned in the absorption area of 600 to 4000 cm-1. The results of analysis consisted of chemical structure, molecular binding form and certain functional groups of tested sample as basic of spectrum type.
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