Dynamic light scattering (DLS)

Instrument & Method: A Malvern Zetasizer Nano ZS (Malvern Instruments Ltd., UK) was used to determine particle size distribution. The analysis was performed using Dynamic Light Scattering (DLS), also known as Photon Correlation Spectroscopy (PCS). DLS measures Brownian motion by analyzing fluctuations in light intensity scattered by particles, correlating this to particle size. The technique is sensitive to particle aggregates, making it useful for assessing nanoparticle stability. Procedure: 0.5 g of the sample was placed in the sample holder and positioned in the analysis chamber. The chamber was cooled to 15°C. On the connected desktop computer, the Dispersion Technology Software v4.00 was launched via the shortcut. Sample information was entered. The nitrogen flow rate was set to 20 (unit assumed to be psi or mL/min). The analysis was started by clicking the Start button in the software.

By Allschoolabs · August 2, 2025 · 79 views

Zetasizer nano-particle analyzer (series Malvern nano ZS) supplied by Malvern instruments ltd., UK was used to determine the size distribution of the given sample. The results were analyzed using standard software Dispersion technology software, version 4.00. The instrument performs size measurements using a process called Dynamic Light Scattering (DLS), also known as PCSPhoton Correlation Spectroscopy, measures brownian motion and relates this to the size of the particles. It does this by illuminating the particles with a laser and analyzing the intensity fluctuations in the scattered light. As DLS is sensitive to the intensity of light scattered by particles, and larger particles scatter more light than small particles, then the DLS is very sensitive to the presence of aggregates, and hence, this technique is an excellent basis for studying the stability of nano particle size distribution. 0.5g of sample was place into the sample holder and the place on the analysis chamber and cover it, on the cheallar and allowed it to cool to 15 degree Celsius then on the desktop computer connected to the equipment and double click on the equipment shortcuts on the desktop computer to allowed you operate the system, input all the information about the sample and Open the nitrogen follow rate 20 finally click on start to start the analysis.